DNA Engine Tetrad 2 Thermal Cycler Operations Manual
5-22 Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
This will add a new temperature step to the protocol as step 2 with a default
temperature of 55.0°C and a duration of 30 seconds.
Note: Steps are always added after the step that is currently selected.
• Select the gradient step (step 1).
• Select <Delete Step>.
The initial gradient step will be deleted, and the newly added temperature
step will be promoted to step one.
Proceed to scenario 3 for instructions on editing step parameters.
3. If the first step displayed is a temperature step, or if a temperature step was
added as indicated in scenarios 1 or 2:
• Position the cursor (text pointer) in the temperature field of step 1 and
select by clicking once.
A flashing insertion point will appear in the temperature field. (See the
“Selecting a Step” and “Editing Step Parameters” sections on page 5-21 for
additional selection and editing options.)
• Use the backspace key on the numeric keypad to delete the current tem-
perature if it is not 94.0°C.
• Enter 94 from the numeric keypad.
• Click once to accept the change.
• Position the cursor (text pointer) in the time:minute field of step 1 and
select by clicking once.
• Use the backspace key to delete the current value if it is not 01.
• Enter 01 from the numeric keypad, and click once to accept the change.
• Position the cursor (text pointer) in the time:second field of step 1 and
select by clicking once.
• Use the backspace key to delete the current value if it is not 00.
• Enter 00 from the numeric keypad, and click once to accept the change.
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