DNA Engine Tetrad 2 Thermal Cycler Operations Manual
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Running Programs
Note: The Operations Chapter (Chapter 4) provides information on running programs with
respect to the operation and use of Alpha units. For example, a program containing a
gradient step will only run in 96-well Alpha units. Please review this chapter for additional
technical detail, to ensure that the program you load and run is an appropriate match for
your Alpha units.
To run a program displayed in either the advanced programming window or the graphical
programming window, select <Save+Run>. Recall that before running a newly created
graphical program, the program must be assigned a name other than the default name,
“TEMPLATE”, by performing a save as. Graphical programs with the default name,
“TEMPLATE”, can not be run.
The Run Program window will appear allowing you to specify the block on which the pro-
tocol should be run, and the calculated control parameters for the run (if applicable) as
described below.
To run preprogrammed or previously created programs (see Appendix C, Factory-
Installed Programsp; Chapter 5, Creating Programs; and Chapter 6, Managing and
Editing Programs), you must first load them into memory.
From the Status window,
• Select <Run> at the bottom of the window or from the <Command>
menu.
• From the open program window that appears, use the external mouse to
select the desired Folder and Program.
Note: the factory programmed protocols are initially stored in the <Main>
folder.
Double check the program listing to ensure that the listed steps are consistent
with the desired program.
• Select <OK>.
You are now presented with the Run Program window.
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