DNA Engine Tetrad 2 Thermal Cycler Operations Manual
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Entering a Goto Step
As currently entered, our program will run one cycle and then end. What we really
want it to do is run 30 cycles total. This involves the insertion of a goto step. Goto
steps are useful for cycling your commands a predetermined number of times. We
have decided to cycle the steps in ADV#1 twenty nine more times.
• Select <GoTo>.
The GoTo Step window appears.
• Position the cursor in the Goto step number field and select.
• Enter 2 in the field.
Note: We do not want to include step one in the cycling process, as this is
our initial denaturation step, and should not be repeated more than once.
• Position the cursor in the Additional Number of Cycles field and select.
• Enter 29 in the field.
• Select <OK>.
The program now appears as follows:
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100°C
1. Incubate at 94°C for 00:01:00
Beep on Target
2. Incubate at 92°C for 00:00:30
Beep on Target
3. Gradient from 45°C to 65°C for 00:03:00
4. Cycle to step 2 for 29 more times
End
Now the program will run with 30 cycles.
As discussed previously, it may be desirable in some cases to ramp to a tempera-
ture at a slower than maximum rate or to include an incremental increase or
decrease in temperature per cycle. Some operators may also wish to include
instructions in a program to open and close a Moto Alpha motorized lid.
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