DNA Engine Tetrad 2 Thermal Cycler Operations Manual
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Opening a Program in Basic Mode
You can choose to open and edit a graphical program in either the graphical pro-
gramming window, or in the advanced programming window. Opening a graphical
program in advanced mode is desirable if you wish to add step modification options,
incubations below 0°C, or other programming features not available in graphical pro-
grams (see the “Types of Programs” section in Chapter 5 for a listing of available
program features). However, once advanced-only features are added to a graphical
program, the program can no longer be opened or edited in basic mode.
We will open our example graphical program, GRAPH#1, in basic mode. The proce-
dure is similar to that described above for opening a program in advanced mode and
is summarized here.
• Select <Programs>.
• Select <Open>.
• Select <Basic Mode>.
The open program window will appear.
• Select the folder FOLDER1.
• Select the program GRAPH#1.
• Select <OK>.
GRAPH#1 will be displayed in the graphical programming window.
Editing a Program
Recall our general program.
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100°C
1. Incubate at 94°C for 00:01:00
Beep on Target (advanced only)
2. Incubate at 92°C for 00:00:30
Beep on Target (advanced only)
3. Gradient from 45°C to 65°C for 00:03:00
4. Cycle to step 2 for 29 more times.
5. Incubate at 10°C forever
End
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