Managing and Editing Programs
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Inserting a Step
Now we will insert an annealing temperature step.
• Highlight step 2.
Recall that new steps are inserted AFTER the highlighted step.
• Select <Temp> from the advanced programming window.
The Temperature Step window appears. Select an incubation Temperature of
60°C, a hold Time of 3 minutes, and the Beep on Target option. Enter param-
eters as described in Chapter 5.
Your program should appear as follows.
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100°C
1. Incubate at 94°C for 00:01:00
Beep on Target
2. Incubate at 92°C for 00:00:30
Beep on Target
3. Incubate 60°C for 00:03:00
Beep on Target
4. Cycle to step 2 for 29 more times.
5. Incubate at 10°C forever
End
Editing a Step
Step 2 of the program ADV#1 includes a 30 second incubation. We wish to change
that incubation to 25 seconds.
• Select Step 2 by double-clicking.
You are presented with the Temperature Step window. The Temperature field
shows 92°C, and the Time field shows 30 seconds.
• Select the Time:Sec field.
• Change the time from 30 to 25.
• Select <OK>.
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