Managing and Editing Programs
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After running the above program, analysis of the resulting sequencing data
indicated that 60°C was the best annealing temperature. We would like to
change step 3 from a gradient step into a temperature step.
Further, step 2 includes a 30 second incubation that we wish to change to 25
seconds.
Editing a Graphical Program
As graphical programming (discussed in Chapter 5) involves essentially editing a
TEMPLATE program, we will only briefly discuss the specifics of editing a preexisting
graphical program here. Please refer to “Entering a Program Using Graphical Mode”
in Chapter 5, specifically, “The Graphical Programming Window” section for more
information.
To replace step 3, the gradient step, with a temperature incubation at 60°C, first open
the program GRAPH#1 in basic mode as described above.
• Select the gradient step.
• Select <Delete Step>.
• Select step 2, the temperature incubation at 92°C.
Recall that new steps are added AFTER the selected step.
• Select <Temp>.
The gradient step has now been replaced with a default temperature step.
Follow the instructions in Chapter 5 for entering temperature step parameters.
To decrease the incubation time of step 2 from 30 seconds to 25 seconds:
• Select the time field of step 2.
• Delete 30 and enter 25 using the numeric keypad.
• Click once to accept the change.
Our example program, GRAPH#1, now appears as follows:
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