BIO RAD Tetrad 2 - Entering a Gradient Step

Creating Programs
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1. Incubate at 94°C for 00:01:00
Beep on Target
2. Incubate at 92°C for 00:00:30
Beep on Target
End
The 92°C denaturation is the first step in the cycling portion of our program.
The next step is the gradient step.
• Highlight Step 2 in the program listing.
• Select <Gradient>.
Entering a Gradient Step
The Gradient Step window indicates the step number and includes fields for specifying
the Lower Temperature and the Higher Temperature of the gradient range as well as the
gradient hold Time. The maximum gradient range is 24°C and the minimum range is
1°C.
Below, the Extend Time option allows for an increase or decrease in incubation time
per cycle, similar to the option available for temperature steps.
Since our hypothetical target is 60°C, we will choose 45°C for the lower limit, and 65°C
for the higher limit. Additionally, we will enter 3 minutes for the incubation hold time.
• Select the Lower Temperature field.
• Enter 45°C.
• Select the Higher Temperature field.
• Enter 65°C.
• Select the Time:Min field.
• Enter 3.