DNA Engine Tetrad 2 Thermal Cycler Operations Manual
7-2 Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
In Chapter 5, we translated an experimental protocol into functional Tetrad 2 programs.
In Chapter 6, we edited program steps. In this chapter, we explore the implementation
of the Tetrad 2 programs, and the instant incubation feature.
Using the Instant Incubation Feature
The DNA Engine Tetrad 2 cycler can also be quite useful as an “instant” constant-temperature
incubator, with a range from –5.0°C to 105°C (4°C to 100°C with the Slide Chambers
™
Alpha
™
unit). This feature can be used for performing ligations, digestions, etc. — or, with slides, per-
forming overnight humidified hybridizations.
Note: The Slide Chambers Alpha unit can be used as a humidified chamber for steady-state
incubations (e.g., hybridizations, color-development reactions). To humidify a block, push one
laboratory tissue into the bottom slot and inject 1 ml of deionized water onto it. See the Slide
Chambers Alpha Unit Operations Manual for complete instructions.
To initiate an instant incubation from the DNA Engine Tetrad 2 cycler’s Status window,
• Select <Instant> at the bottom of the window or from the <Command>
menu.
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