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BIO RAD Tetrad 2 - Page 74

BIO RAD Tetrad 2
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Creating Programs
Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com 5-37
The final program should appear as follows:
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100°C
1. Incubate at 94°C for 00:01:00
Beep on Target
2. Incubate at 92°C for 00:00:30
Beep on Target
3. Gradient from 45°C to 65°C for 00:03:00
4. Cycle to step 2 for 29 more times.
5. Incubate at 10°C forever
End
The program is now finished. Select <Save> to ensure that your work is preserved.
Chapter 6 will explain how to edit the various programming steps to include
different parameters and Chapter 7 will instruct you on how to run this program.

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