Operating Instructions RF-2000
69
1) Spiky noises are observed.
2) The base line drifts in one direction and abruptly changes its drift
direction. This symptom appears repeatedly.
3) The base line is not stabilized. Excessive drifts or noises are
observed.
When the end of the flow cell outlet pipe is blocked, the pen moves
greatly.
(2) Checking the flow cell whether bubbles are present inside or not.
Remove the flow cell from the sample compartment, and hold the cell holder
toward a light source (light bulb, fluorescent lamp, etc.) Look into the flow cell
located in the cell holder and check the upper and lower side of the cell
whether any bubbles are present inside or not.
(1) Flow cell (2) Teflon packing
Example : Bubbles in the flow cell
8.1.8 Driving bubbles out and liquid leakage test
(1) Remove the flow cell from the instrument.
(2) Flow methanol or acetonitrile into the flow cell using a syringe or a pump. The
flow rate should be as high as possible.
(3) Ensure that no liquid leaks from the flow cell. If leakage is observed, stop the
liquid flow, and make an appropriate measure according to section "8.1.4
Liquid leakage from flow cell".
(4) Visually check the flow cell light path, and check whether bubbles are present
or not. If any bubbles be trapped inside the cell, stop and open the outlet
tubing lightly and repeatedly with finger to flush away the bubbles.
If bubbles cannot be driven out of the flow cell, drain the liquid from the flow
cell once and fill the liquid into the flow cell again, and check the cell for
bubbles. If bubbles are still present in the flow cell, repeat these procedures
several times.
(5) When no more bubbles are present, mount the flow cell to the equipment.
(6) If bubbles cannot be removed by above procedures, the inner surface of the
flow cell may be stained. Drain the liquid from the flow cell, and then clean the
flow cell according to Sections "8.1.2 Simple Cleaning of flow cell" and "8.1.3
Cell disassembling and cleaning".
Please note:
When a mobile phase which is immiscible with methanol or
acetonitrile is used, replace inside the flow paths with a liquid
which is miscible first. When a buffer is used, flow distilled
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