Eppendorf 5702 - 5.5 Loading a swing-bucket rotor; 5.5.1 Inserting the bucket in the swing-bucket rotor; 5.5.2 Performing an imbalance calibration

Operation
Centrifuge 5702/5702 R/5702 RH
English (EN)
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5.5 Loading a swing-bucket rotor
5.5.1 Inserting the bucket in the swing-bucket rotor
Prerequisites
• The combination of rotor, bucket, adapter and vessel has been approved by Eppendorf.
• Buckets that are located opposite each other belong to the same weight class. The weight class is
engraved in the sides of the groove: e.g., 68.
• The bucket grooves are clean and have been lubricated slightly with pivot grease.
1. Check the maximum load (adapter, vessel, and contents) for each bucket. Check the length of the
vessels.
The weight that a fully loaded bucket must not exceed is indicated on each rotor.
2. Insert the buckets into the rotor. Load the rotor symmetrically
All rotor positions must be occupied with buckets.
Only place buckets opposite each other that have the same weight class.
3. Check that all carriers are hanging properly and can swing freely.
5.5.2 Performing an imbalance calibration
You can perform a manual imbalance calibration to check how the buckets are swinging. The speed of
centrifugation must be a maximum of 1000 rpm.
Perform the imbalance calibration when the following prerequisites have been met:
• You are using the tubes for the first time.
• You are using tubes with a length of > 100 mm.
1. Bucket loaded with tubes.
2. Equip the rotor with buckets.
3. Accelerate the rotor manually until the buckets swing out at 90°.
If the following results are obtained the imbalance calibration was successful:
• The buckets swing freely.
• The tubes do not touch the rotor cross.
CAUTION! Risk of injury due to asymmetric loading of the rotor.
 Load rotors symmetrically with identical tubes or plates and buckets.
 Always load all positions of a swing-bucket rotor with buckets.
 Only load adapters with suitable tubes or plates.
 Always use tubes or plates of the same type (weight, material/density and volume).
 Check that loading is symmetrical by balancing the adapters and tubes or plates used with
scales.
 Carry out an imbalance calibration if you are using tubes or plates for the first time.