Implen NanoPhotometer NP80 - Page 5

NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
5
NANOVOLUME MULTI SAMPLE MEASUREMENT BASICS (N120) ........................................................... 49
CUVETTE MEASUREMENT BASICS (C40/NP80) .................................................................................. 51
SUBMICROLITER CELL MEASUREMENTS BASICS (C40)....................................................................... 52
SAMPLE HANDLING TIPS ................................................................................................................... 53
SOLVENT COMPATIBILITY (N50/N60/N120/NP80/C40 WITH SUBMICROLITER CELL) ............................. 55
DATA TRANSFER VIA FILE SERVER .................................................................................................... 56
BATTERY OPERATION ............................................................................................................ 57
4. NANOPHOTOMETER
®
APPLICATIONS ................................................................... 58
NUCLEIC ACIDS .................................................................................................................. 58
METHOD OVERVIEW .......................................................................................................................... 58
MEASUREMENT PROTOCOL SINGLE SAMPLE...................................................................................... 58
MEASUREMENT PROTOCOL MULTI SAMPLE (N120) ............................................................................ 62
CALCULATIONS ................................................................................................................................ 65
PROTEIN UV ...................................................................................................................... 70
METHOD OVERVIEW .......................................................................................................................... 70
MEASUREMENT PROTOCOL SINGLE SAMPLE...................................................................................... 71
MEASUREMENT PROTOCOL MULTI SAMPLE ....................................................................................... 74
CALCULATIONS ................................................................................................................................ 78
PROTEIN ASSAYS ............................................................................................................... 84
METHOD OVERVIEW .......................................................................................................................... 84
MEASUREMENT PROTOCOL SINGLE SAMPLE...................................................................................... 85
MEASUREMENT PROTOCOL MULTI SAMPLE ....................................................................................... 88
SAVING AND LOADING STANDARD CURVES ........................................................................................ 93
CALCULATIONS ................................................................................................................................ 93
KINETICS .......................................................................................................................... 94
METHOD OVERVIEW .......................................................................................................................... 94
MEASUREMENT PROTOCOL ............................................................................................................... 94
CALCULATIONS ................................................................................................................................ 96
OD600 .......................................................................................................................... 97
METHOD OVERVIEW .......................................................................................................................... 97
MEASUREMENT PROTOCOL ............................................................................................................... 98
CALCULATIONS .............................................................................................................................. 100
MORE APPS ..................................................................................................................... 101
MORE APPS: WAVELENGTH .............................................................................................. 101
METHOD OVERVIEW ........................................................................................................................ 101
MEASUREMENT PROTOCOL ............................................................................................................. 101
CALCULATIONS .............................................................................................................................. 104
MORE APPS: WAVESCAN ................................................................................................. 105
METHOD OVERVIEW ........................................................................................................................ 105
MEASUREMENT PROTOCOL SINGLE SAMPLE.................................................................................... 105
MEASUREMENT PROTOCOL MULTI SAMPLE ..................................................................................... 107
CALCULATIONS .............................................................................................................................. 110
MORE APPS: ABSORBANCE RATIO ................................................................................... 111