6. Application Example
53
Design Layout
Define the plate layout using the Well Assignment dialog box on the right side of
the screen.
In the Identifiers group box, select BL (Blank).
In the Experimental group box the number 1 remains.
In the Replicates group box, All is selected automatically.
Click well A1, which is then marked with a red border.
Click Fill selection and the well is labeled with the selected identifier type.
Note
A single well can also be filled by double-clicking it.
Now choose the following settings in the Well Assignment dialog box:
In the Identifiers group box, select NC (Negative Control).
In the Experimental group box the number 1 remains.
All in the Replicates group box is selected automatically.
Starting at well B1 click and drag the mouse to C1. The wells B1 to C1 are then
marked with a red border.
Click Fill selection and the wells are labeled with the selected identifier type.
Calibrators (standards) must be assigned to wells D1 to G2. Select the following
settings in the Well Assignment dialog box:
In the Identifiers group box, select ST (Standard).
In the Experimental group box the number 1 remains.
In the Replicates group box, choose between Fix number and All.
Fix number:
Only enabled for standards and samples where IDs can be used.
If this Fix number button is active a number can be entered in the corresponding
text field. This number defines how many replicates are intended for this method.
In the selected wells, the entered number of replicates for every ID is created.
Therefore the number of selected wells must be a multiple of the entered number
of replicates.
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