Nexcope NIB620 - Main Body Suitable for Clean Bench; Bright Field Settings According to Koehler; Prerequisites for Setting Koehler Illumination

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4.3.10. Main body suitable for Clean Bench
Illustration 051: NIB630 - Microscope body suitable for Clean Bench.
The body is compact, stable and suit-
able for a Clean Bench:
Can be sterilized in the Clean Bench
On the premise of ensuring the effect of
imaging, NIB630 is with compact design.
The volume and weight of the body is re-
duced as much as possible in principle of
stablity. The compact body is with anti-UV
coating and can be placed into the Clean
Bench for sterilization under UV lamp.
Cell sampling and operation can be
performed in Clean Bench
The distance between the eye point to the
operation button and the focusing knob is
relatively short, and the distance from the
stage is far away. It is available to make the
viewing head and operating mechanism out-
side, and stage, objectives and sample in-
side. So realize cell sampling and operation
inside and observing comfortably outside.
4.4. Bright field settings according to Koehler
4.4.1.
Prerequisites for setting Koehler illumination
1
2
5
3
4
Illustration 052: NIB630 - Components of the Koehler illumination.
Condenser (1)
• Height adjustable via the condenser up-
down knob (2)
• Centreable via condenser centering screws
(3)
Aperture diaphragm
• Adjustable via the aperture diaphragm lever
(4)
Field diaphragm
• Adjustable via the field diaphragm lever (5)
Condenser task: filling the entire aperture (the opening width) of the objective with
light in order to achieve the greatest possible resolution.
With the aperture diaphragm you can control the resolution, contrast and depth of
field. After each change of objective, the aperture diaphragm must be readjusted.
When the illumination is set correctly, only the section of the sample being examined
is illuminated by the field diaphragm. The sample is protected from excessive light irra-
diation and at the same time the formation of contrast-reducing stray light is minimised.
Always set the Koehler illumination on the NIB630 microscope with the sample
carrier/sample bottle that will subsequently be used.
NIB630
4
Example based on NIB620.