Nexcope NIB620 - Emboss Contrast Observation (Relief 3 D Contrast); Overview; Oblique Illumination; Components for Emboss Contrast Observation

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OBSERVATION METHODS
5.3. Emboss contrast observation (Relief 3D contrast)
5.3.1. Overview
Emboss contrast is a newly developed contrast observation method which
is easy to operate. Even without extra optical components, pseudo-three
dimensional and glare-free image can be optained just through adding
adjustment slider. This technique allows the observation of thick cells such
as embryos or iPS cells (induced pluripotent stem cells) that would normally
suffer from halos in conventional phase contrast methods. Emboss contrast
is compartible with glass and plastic culture dishes.
5.3.2. Oblique illumination
The emboss contrast observation is based on the method of oblique illumi-
nation. Here, a gain in contrast is achieved by covering part or half of the
normal illumination beam path. The condenser-side emboss contrast slider
is equipped with a sector diaphragm.
This blocks part of the light. The specimen is then illuminated at an angle,
resulting in a shadow cast on the fine structures of the specimen. The eye-
piece-tube-side emboss contrast slider serves as a ring diaphragm. This cuts
the light beam out of the light cone.
5.3.3. Components for emboss contrast observation
All components for emboss contrast observation are not included in the scope of
delivery. This set can be purchased as an accessory.
The emboss contrast observation set consists of two emboss
contrast sliders:
3
2
1
Illustration 59 A: NIB610/NIB620/NIB630 -
Condenser-side emboss contrast slider.
40x
Bright field position marking 10x position marking
20x position marking
40x position marking
20x
10x
Illustration 59 B: NIB610/NIB620/NIB630 -
Eyepiece-tube-side emboss contrast slider.
Condenser-side emboss contrast slider
The slider matches with the 10x/20x/40x
phase contrast objectives.
• The slider is equipped with a sector
diaphragm (1)
The specimen is then illuminated at an
angle
• Green filter (ND6 filter) (2)
Suppresses disturbing color fringes on
the object contours
• Hollow positions (3)  position without
ring diaphragm  bright field observation
Eyepiece-tube-side emboss contrast
slider
The slider has position markings for emboss
contrast microscopy.
serves as a ring diaphragm; this cuts the
light beam out of the light cone; sharp
edges are created