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5
OBSERVATION METHODS
5.2.3. Settings for phase contrast observation
NIB630
A
66
33
22
77
44
55
11
Illustration 58 A: NIB630 - Settings before you start.
CT
B
3
4
2
1
Illustration 58 B: NIB630 - Settings for phase contrast.
5
6
7
C
Overlap
Illustration 58 C: NIB630 - Settings for phase contrast.
Before you start...
1. Microscope is switched on
2. Transmitted light ON
3. Changing lever for visual observation/
photo is set to binocular part
4. Use the transmitted light path shutter
lever to open the shutter.
5. Phase contrast slider is set to bright field
6. 10x objective is swivelled in
7. Object is in focus
Unit is set in bright field according to Koehler
Set the phase contrast slider (1) to ring
diaphragm 10/20/40.
Set the aperture diaphragm lever (2) all the
way to the left so that the aperture of the
condenser is fully opened.
If the aperture diaphragm is
closed, it hinders the ring diaphragm
and phase contrast effects cannot be
obtained.
Replace an eyepiece (3) with the centering
telescope (CT) (4).
Use a suitable hexagonal
screwdriver.
The CT enlarges the phase rings
and makes centering easier. An
approximate centering of the phase
rings is also possible without CT.
Turn the CT until two sharp rings become vis-
ible. Check the centering and overlap of the
bright ring diaphragm (in the condenser) (5)
with the dark phase ring (in the objective) (6).
Adjust the centering screws (7) on the phase
contrast slider until the bright ring diaphragm
and the dark phase ring completely overlap.
Use a suitable hexagonal
screwdriver.
Perfect phase contrast can only
be achieved when the bright ring
diaphragm and the dark phase ring
exactly covers the beam path.
Then switch to the next objective, check the
phase rings for all objectives and adjust if
necessary.
After centering, remove the CT, insert the
eyepiece and observe your sample in phase
contrast.
When observing and replacing the speci-
men, check the focus and the brightness
adjust them as necessary.
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