Nicoya OpenSPR-XT - Page 71

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If the buffer/air interface is being drawn in through the tubing, and buffer is seen entering the
flow cell, then your problem is likely a leak from the flow cell at the Fluidics Block– see section
below to resolve. If there are no leaks at the Fluidics Block, check for leaks at the Outlet Port and
try removing and replacing the Outlet tubing. If following these steps does not resolve the issue,
it is an indication of a clog in the outlet tubing – contact support@nicoyalife.com for further
assistance.
If the buffer/air interface is being drawn in through the tubing, and buffer is not seen entering
the flow cell, inspect the inlet to the flow cell and the respective barbed fitting and remove any
obstructions using a syringe tip or tweezers. If no obstructions are present and flow is not
restored, an internal leak or tubing rupture is the likely cause. Turn off your system immediately
and contact support@nicoyalife.com .
If the buffer/air interface is not being drawn in through the inlet tubing, there is likely a clog in
the tubing. Contact support@nicoyalife.com for further assistance.
Leaks at Fluidics Block: The presence of a leak can be signaled by liquid pooling around the edges of
the sample holder. It can also be monitored by a lack of liquid coming from the Flow Cell Outlet. A
persistent decrease in the baseline with no stabilization over time could also signify a slow leak. The
user must be careful of leaks as the OpenSPR-XT™ is not waterproof. When a leak is suspected, check
to see if the sample holder is properly aligned and seated tightly. If so, disassemble the sample holder
to see if the Flow Cell is seated properly and if there are tears in the Flow Cell material near the Inlet
and Outlet. If the Flow Cell appears to be seated properly and there are no visible tears, dry all the
components with N
2
gas or compressed air, reassemble and run buffer through. If the leaks continue,
replace the Flow Cell. Also, check the fluidic connections at the Inlet and Outlet.
Real time signal jump: These sometimes occur when the system is knocked. Allow enough time to
pass for a new baseline to be formed before continuing with the experiment and try not to disrupt the
system when making injections. Check for bubbles and leaking as this may also cause real time signal
jumps.
If the preceding troubleshooting suggestions do not work or does not cover your difficulty, contact
Nicoya customer service at support@nicoyalife.com and one of our technicians will gladly help.