Chapter 5. Capturing Multistained Images: Cross Talk Reduction (Standard Detector) A1
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(5) Click the [Live] button and adjust [Laser] (laser power) and [HV] (detector sensitivity) while
checking the image.
Note: Use Offset “0” as the standard setting.
Note: Using the
[Pixel Saturation Indication] button in the LUTs tab during adjustment makes it
easy to adjust the sensitivity.
Note: If the displayed image is dark, click the [Auto Scale All] button to adjust the contrast of the
channel automatically to make the image clear.
Note: Turning on and off the [Live] button (scan ON/OFF) during adjustment minimizes fading.
Note: If the LUTs tab is not displayed, right-click the gray area of the software and select
[Visualization Control] - [LUTs] from the displayed menu to call it.
Click the [Split Channels] button to
display channels in division which facilitates
adjustment of each channel. Reclicking the
button returns to the previous display.
Using the [Pixel Saturation Indication] button during
adjustment colors the saturation region.
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