Nikon A1 User Manual

Chapter 9. Capturing Z Series Images (While Changing Brightness) A1
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9.4 Register laser power, HV, or others at each Z position.
(1) Check the [Move Z to selected Point]
checkbox and double-click the Z position in
the Z [μm] column to move the focus to the
displayed Z position.
(2) Check the [Use on Live] checkbox under [Live
Correction].
When this checkbox is checked, changing the
Z position displays the Live image with the set
HV and laser power.
After you finish checking, uncheck the
checkbox.
(3) After the Z position is moved to where you want to adjust brightness, adjust HV and laser power.
(4) To register the adjusted HV and laser power, click
.
After you finish adjusting brightness at each Z position, you can close [Z Intensity Correction].
(5) After you finish all registrations, click the [Run
Z Corr] button in the Capture Z-Series dialog
box to start image acquisition.
(3)
(4)
(2)
(1)

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available