Nikon A1 User Manual

Chapter 19. Capturing Spectral Images (Spectral Detector) A1
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19.5 Set the optical path. (Optical path setting for the confocal system required for
acquiring images)
Check the settings.
(1) Click
to open the Optical path window.
(2) Click the
[SD] button to select the spectral detector.
(3) Click the
[Manual] button to set the optical path in the manual mode.
(4) Check the checkboxes of the channels of lasers to be used.
(5) Select a value for [Resolution] (wavelength resolution to be used) from “2.5 nm”, “6 nm”, and “10 nm” and a
value for [Channels] (number of channels to be used) from “1” to “32”. Set the wavelength band to be acquired
by shifting the bar.
(6) Select “BS20/80” for [1st DM].
(7) If acquiring a transmitted image together with a spectral image, click
to bring into the optical path.
Note: Before acquiring a transmitted image, turn off the light above the microscope.
Note: Because the transmitted light detector is placed in front of transmitted light, transmitted images
(differential interferences (DIC)) cannot be observed visually while putting the transmitted light
detector into the optical path.
To observe transmitted images visually, remove the transmitted light detector from the optical path.
(2)
(3)
(4)
(5)-1
(6)
(7)
(5)-2
(1)

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available