Chapter 19. Capturing Spectral Images (Spectral Detector) A1
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(1) If you want to acquire a transmitted image together with a spectral image, click the TD [IN] button and
check the TD checkbox.
Note: Before acquiring a transmitted image, turn off the light above the microscope.
(2) Select the laser wavelength to be used from [Pinhole].
Select a pinhole size best suited for the objective with [▲Home
].
(3) Click the
[Live] button and adjust [Laser] (laser power) and [Si HV] (detector sensitivity) while
checking the image.
Note: The Si HV setting is common to all lasers. Make fine adjustments with the laser power setting.
Note: Using the [Pixel Saturation Indication] button in the LUTs tab during adjustment makes it
easy to adjust the sensitivity.
Note: If the displayed image is dark, click the
[Auto Scale All] button to adjust the contrast of the
channel automatically to make the image clear.
Note: Turning on and off the [Live] button (scan ON/OFF) during adjustment minimizes fading.
Note: If the LUTs tab is not displayed, right-click the gray area of the software and select
[Visualization Control] - [LUTs] from the displayed menu to call it.
(4) To use Auto Gain, a function that automatically adjusts the detector sensitivity (HV) based on the set rate
of saturated pixels, click the
[AG] button.
“NG” is displayed for channels that failed in Auto Gain and the HV values are returned to the previous
values.
Use the
[Auto Gain setting] button to change the rate of saturated pixels. Set the maximum value
and minimum value of the rate.
Notes:
• Auto Gain is disabled during scanning.
• Auto Gain is disabled when line scan is set.
• Do not make a manual adjustment in the Acquisition window and do not make an adjustment
using the remote controller during Auto Gain.
Using the [Pixel Saturation Indication] button during
adjustment colors the saturation region.
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