Chapter 22. Capturing Virtual Filter Images (Spectral Detector) A1
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22.6 Determine image acquisition conditions.
(1) Select the laser and channel to be used.
(2) If you want to acquire a transmitted image together with a spectral image, click the TD [IN] button and
check the TD checkbox.
Note: Before acquiring a transmitted image, turn off the light above the microscope.
(3) Select the laser wavelength to be used from the pull-down menu of [Pinhole].
Select a pinhole size best suited for the objective with [▲Home
].
(6)-1 Selecting resolution
(6)-3 Selecting
scan speed
(6)-2 Laser application
time per pixel
(8) Acquisition
(4)-1 Live (Starting scanning)
(4)-2 Adjusting laser power and
gain
(2)-2 Selecting a
transmitted image
(2)-1 Selecting a transmitted image
(1) Selecting
lasers
(3) Selecting
a pinhole
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