Nikon A1 User Manual

Chapter 25. Capturing High-Speed Images (Resonant Scanner) A1
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(2) Select the laser and channel to be used.
(3) If you want to acquire a transmitted image together with a confocal image, click the TD [IN] button and
check the TD checkbox.
Note: Before acquiring a transmitted image, turn off the light above the microscope.
(4) Select the laser wavelength to be used from [Pinhole].
Select a pinhole size best suited for the objective with [▲Home
].
(5) Click the
[Live] button and adjust [Laser] (laser power) and [HV] (detector sensitivity) while
checking the image.
Note: Use Offset “0” as the standard setting.
Note: Using the [Pixel Saturation Indication] button in the LUTs tab during adjustment makes it
easy to adjust the sensitivity.
Note: If the displayed image is dark, click the
[Auto Scale All] button to adjust the contrast of the
channel automatically to make the image clear.
Note: Turning on and off the [Live] button (scan ON/OFF) during adjustment minimizes fading.
Note: If the LUTs tab is not displayed, right-click the gray area of the software and select
[Visualization Control] - [LUTs] from the displayed menu to call it.
Using the [Pixel Saturation Indication] button during
adjustment colors the saturation region.
A
uto Scale All

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available