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Nikon A1 User Manual

Nikon A1
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Chapter 3. Operation of the Microscope A1
9
3.3 Operation Panel on the Microscope Ti-E Main Body
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
Right operation panel
Coarse
Fine
ExFine
Obj.
ON/OFF
6V30W
MAX.MIN.
12V100W
Left operation panel
Other buttons on the panel can be operated from the Ti remote control pad.
3.4 HG Controller Operation (Shutter Controller for Visual Fluorescence Microscopy)
(1) Press the SHUTTER button to open the
shutter and start fluorescence microscopy.
(Open: LED lights, Close: LED lights out)
(2) If the sample is severely faded, press the ND
select up button to reduce the excitation light
and start microscopy.
ND values are in the range from 1 to 32.
The larger the value, the darker the
excitation light becomes.
(3) Press the SHUTTER button to close the
shutter and finish the fluorescence
microscopy.
LAMP OPEN
C-HGFIE
HG CONTROLLER
POWER
SHUTTER
ND
32168421
Refocus switch
(objective refocusing)
Escape switch
(objective retraction)
Coarse/fine motion select
switch
Focus knob
(common to coarse motion
and fine motion)
Coarse/Fine/ExFine
switch/indicator
(switches/displays
focusing knob resolution)
Focus knob
Brightness control knob for the
diascopic illumination lamp
Diascopic illumination lamp
ON/OFF switch
Obj. switch
(objective switch)
SHUTTER button
(toggles between
open and close
when pressed)
ND select buttons
(six steps, up/down)
ND indicator

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Nikon A1 Specifications

General IconGeneral
TypeConfocal Microscope
SoftwareNIS-Elements
Laser Lines405 nm, 488 nm, 561 nm, 640 nm
DetectorGaAsP PMT detectors
Imaging ModesConfocal, Spectral, FRAP
Scanning MethodGalvanometer-based scanning
ObjectivesCompatible with Nikon CFI objectives
DimensionsVaries with configuration
WeightVaries with configuration
LaserMultiple laser lines available

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