Nikon A1 User Manual

Chapter 3. Operation of the Microscope A1
8
3.2 Microscopy Setting
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT
DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z
-
RESET
1X
1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
PFS
DOWN
UP
PFS
OFFSET
CONTROLLER
FINE
COARSE
LAMP OPEN
C-HGFIE
HG CONTROLLER
POWER
SHUTTER
ND
32168421
Diascopic
microscopy (DIC)
Fluorescence
microscopy
Confocal microscopy
Optical path E100 E100 L100
Fluorescent filter ANALY DAPI, etc Blank position
Condenser N1 or N2
Unnecessary
(No problem if provided)
Unnecessary
(No problem if provided)
- Necessary when acquiring a DIC
image using a laser
Ti control pad*
Diascopic illumination On Off Off
HG controller Fluorescent shutter Off On Off
Polarizer Necessary
Unnecessary
(No problem if provided)
Unnecessary
(No problem if provided)
- Necessary when acquiring a DIC
image using a laser
Microscope
D filter
NCB filter
ND filter
Necessary
Unnecessary
(No problem if provided)
Unnecessary
- Be sure to remove it when acquiring
a DIC image using a laser
*: Can be called collectively using the function button on the Ti control pad.
D filter
ND filter
Polarizer
Slide to put it into optical path.
Field diaphragm
knob
Aperture diaphragm open/close leve
r
Condenser centering knobs (both sides)
Condense
r
Condenser lens
Eyepiece tube (Figure: TI-TD)
Eyepieces (both sides)
Eyepiece base unit
(Figure: TI-T-B)
Power suppl
y
(Figure: TI-PS100W/A)
Microscope base (Figure: Ti-E)
Optical path
Front operation panel
Diascopic illumination
Nosepiece (Figure: TI-ND6-PFS)
Condenser focus knobs (both sides)
Dia illumination lamphouse
(Figure: D-LH/LC)
NCB filter
GIF filter
Filter sliders
(both sides, four in total)
Condenser mount
Dia pillar illuminator (Figure: TI-DH)
Objectives
Stage ring (annular ring)
Stage (Figure: TI-SR)
Fluorescent filter
Right side port
*
Stage knob
Right connector panel
PFS Offset
Controller
Focus knob
Right operation panel
SHUTTER button (toggles between
open and close when pressed)
ND select buttons (six steps,
up/down)
Fluorescent shutter
ND indicator

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available