Instrument Methods . 69
11. Enter the volume of Diluent by using the keypad numbers.
The available range is from 0.01 to 9999.
12. Press OK
to calculate the dilution factor and return to the Method Settings screen.
13. Select the Units of measurement using the left and right arrows, and then press the
down arrow.
The options are µg/ml, ng/µl, µg/µl.
14. Enter the Factor using the keypad numbers and then press the down arrow.
The available range is from 0.001 to 9999.
15. Press OK
to start measurements.
The Absorbance Ratio results screen is displayed.
16. Insert the reference sample and then press
.
This reference measurement will be used for all subsequent samples until repeated.
17. Insert your first sample and press
.
The absorbance at each wavelength is measured. If Background was selected, the
background absorbance is then subtracted from the absorbance at Wavelength 1 and
Wavelength 2. The ratio between Wavelength 1 and Wavelength 2 is calculated.
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