ADVANCED FEATURES
PerkinElmer Life and Analytical Sciences 147
To establish the optimal region settings for a nuclide, you will need to perform the
following tasks:
1. Load the background vial in an odd numbered cassette position and the
sample vial in even numbered cassette positions.
Note:A background vial must be counted for each sample vial.
Attach the Priostat flag in the reset position to the cassette.
2. Select Low Level Optimize from the Run Menu. The Sample Priostat window is
displayed.
3. Select Count from the Run menu. Counting will commence immediately after
the instrument identifies the Priostat flag.
4. Allow the samples to count long enough to reach statistical accuracy (2S%) of
2.00 (equivalent to 10,000 gross counts) before terminating the counting
procedure. The recommended minimum count time for a background sample is
ten minutes.
5. Stop the counting of a sample by selecting the Run-Stop menu item.
6. Select Next Sample followed by Count from the Run menu, if you would like to
count additional samples.
7. Stop the Low Level Optimize counting procedure by selecting the Run-End
Sample Priostat menu item. An output window is displayed which provides you
with the optimal region settings for the sample nuclide.
8. Add this nuclide with the optimized region settings into the Sample Nuclide
Library for later use in various assays. Any quench standards sets defined for
use with this nuclide will automatically use the new region settings. No new
quench standards need to be counted as long as the nuclide is the same.
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