CHAPTER 2
26 PerkinElmer Life and Analytical Sciences
How To Set Up a Quench Standards Assay
The following tasks are required when performing a Quench Standards Assay.
This requires the single/dual/color DPM option on the 2800TR.
1. Calibrate the instrument, if necessary.
2. Create a new assay, choosing Quench Standards as the assay type and define
the new assay parameters.
Or
Open an existing Quench Standards Assay and edit or review if necessary.
3. Save the assay in the Assays folder of the Packard\TriCarb directory.
4. Associate (link) the assay parameters with a protocol and attach the
corresponding protocol flag to the first cassette to be counted.
5. Load the cassette(s) with vials and load the instrument with cassettes.
6. Start the instrument.
Note:When using the Low Level count mode (if available), you
must NOT use quench standards which have been purged free of
oxygen with an inert gas. The oxygen quenching in unpurged
standards facilitates discrimination between background and true
beta events. Unpurged standards are available from PerkinElmer
Life and Analytical Sciences.
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