ASSAYS
PerkinElmer Life and Analytical Sciences 67
Chapter 5
Assays
CPM Assays
A CPM assay provides you with information regarding the total quantity of
radioactivity within a sample, in one, two or three predefined counting regions.
The data generated is expressed in CPM (Counts Per Minute). CPM reflects only
the activity that is detected, without regard to counting efficiency or sample
interference, such as quench.
Performing a CPM Assay
The following tasks are required when performing a CPM Assay.
Calibrate the instrument, if necessary.
Create a new assay, choosing CPM as the assay type and define the new
assay parameters, OR open an existing CPM assay and edit or review if
necessary. Save the assay in the Assays folder of the Packard\TriCarb
directory.
Associate (link) the assay parameters with a protocol and attach the
corresponding protocol flag to the first cassette to be counted.
Load the cassette(s) with vials and load the instrument with cassettes.
Start the instrument.
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