CHAPTER 5
84 PerkinElmer Life and Analytical Sciences
Count Time
Enter the maximum length of time that the samples will be counted. For low
activity samples, longer count times provide better count statistics and more
accurate sample results. Changes to this parameter for an active assay will be
implemented immediately.
Assay Count Cycles
Enter the number of times you would like the assay to count. The assay is
recounted after it has moved one complete cycle around the sample changer
deck. Any samples on the sample changer deck will be counted prior to your
samples being recounted.
Repeat Sample Count
Enter the number of times you would like each sample counted while in the
detector. This differs from Assay Count Cycles, where samples are unloaded from
the detector and make a complete cycle around the sample changer deck prior to
recounting.
Calculate Percent Reference
Activate the percent reference calculation by marking this box. The instrument
reports the value of each sample as a percentage of a reference vial. The
reference vial should be the first non-background vial loaded in the cassettes.
Number of Vials per Sample
Enter the number of replicates of each sample being counted. The data output
will report the average value of the replicates.
Lower Limit Regions A, B, and C
These fields represent the lower counting limit for regions A, B, and C, measured
in keV.
Note: This field will only be enabled for Single Label DPM Assays
where tSIE is selected as the Quench Indicating Parameter. The
system uses the tSIE and the sample spectrum endpoint to
determine sample heterogeneity.
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