Vers.: 20230710ENG
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3 Understanding the system principles
3.1 Introduction
The measurement methods used in this analyzer are: sheath flow impedance method for
determining the RBC and PLT data; colorimetric method for determining HGB concentration
and SF Cube Cell Analysis technology (laser flow cytometry and fluorescent staining) for
determining the WBC differential data and the RET data. Other parameter results are obtained
via calculation.
3.2 WBC measurement by the SF Cube Cell Analysis technology
In normal peripheral blood, WBC can be classified into five categories: lymphocytes,
monocytes, neutrophils, eosinophils and basophils. However, under the influence of certain
diseases, the peripheral blood may contain various abnormal cells apart from the five
subpopulations of normal cells, such as atypical lymphocytes, immature cells, etc. Most of
these abnormal cells are different kinds of immature cells in the cell generation process.
Nevertheless, they have in common a great deal of nucleic acid (DNA and RNA), the content
of which decreases as the cell gets more mature. Therefore, normal cells and immature cells
can be differentiated by detecting the content of nucleic acid in the cells by using a fluorescent
staining and a laser beam: the SF Cube Cell Analysis technology.
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