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Fluidic Connections 23
Required Materials
• 5/16-inch open-end wrench
• 0.009-inch (0.23 mm) I.D. stainless steel tubing (in Startup Kit)
• Stainless steel tubing cutter or scribing file
• Pliers, plastic-covered, or with cloth
• Compression screw assembles, three
Procedure
To make fluidic connections to the 996 detector:
1. Measure the lengths of tubing needed to connect:
• The column outlet to the 996 detector inlet.
Note:
Be sure that you keep the length of this tubing as short as possible to
prevent band broadening.
• The 996 detector outlet to a waste collection bottle.
Note:
Ensure the length of this tubing is at least 1 to 2 feet (30 to 60 cm) to
prevent air bubbles from forming in the flow cell.
2. Cut the two lengths of tubing as follows:
• Use a Waters 1/16-inch stainless steel tubing cutter or a file with a cutting edge
to scribe the circumference of the tubing at the desired break point.
• Grasp the tubing on both sides of the scribed mark with cloth- or
plastic-covered pliers (to prevent marring the surface), then gently work the
tubing back and forth until it separates.
• File the tubing ends smooth and straight to minimize dead volume and band
broadening.
3. Assemble a compression fitting (as shown in Figure 1-7
) at both ends of the
column outlet line and at one end of the detector outlet line.
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