34 Maintenance
3
To flush the flow cell:
1. Select a solvent compatible with the samples and mobile phases that you have been
using. If you have been using buffers, flush with HPLC-grade water for 10 minutes at
1 mL/min, then switch to a low-surface-tension solvent such as methanol.
Attention:
Ensure that the solvent is miscible with the previous mobile phase.
2. Set pump flow to
1
mL/min, then run the pump 10 minutes.
3. Test the lamp energy by performing the Lamp diagnostic test. For details, refer to
the “PDA Diagnostics Window” topic in the
Millennium
32
Online Help
Find tab.
If the lamp diagnostic fails and the lamp has not been used more than 1000
hours, disassemble the flow cell and clean the flow cell components using the
procedure described in Section 3.1.2,
Removing the Flow Cell.
3.1.2 Removing the Flow Cell
Note:
You do not need to shut down the 996 detector to remove and replace the flow cell.
Required Materials
• 5/16-inch open-end wrench
• Phillips screwdriver
Procedure
To remove the 996 detector flow cell:
1. Set the flow to
0.0
mL/min.
2. Power off the solvent delivery system or pump.
Caution:
To avoid the possibility of leaking mobile phase, do not disconnect the
inlet or outlet fluidic lines while there is pressure in the chromatographic system.
Always vent your system before disconnecting fluidic lines.
3. Use the 5/16-inch wrench to disconnect the fluidic lines at the front of the
detector.
4. Lift up the 996 detector front cover and pull the front cover from the detector
chassis.
5. Open the flow cell access door by pulling the black thumbtab, then pull the door
gently toward you (Figure 3-1
).
STOP
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