Waters 996 - Effects of Ph and Solvent Concentration on the Absorbance Spectrum of P -Aminobenzoic Acid

62 Spectral Contrast Theory
5
Figure 5-6 Effects of pH and Solvent Concentration
on the Absorbance Spectrum of
p -Aminobenzoic Acid
Wavelength (nm)
200 220 240 260 280 300 320 340
pH 6.9
pH 5.1
pH 3.1
NH
+
3
NH
2
NH
2
COOH
COOH
Effect of pH
p - Aminobenzoic Acid
COOH
200 220 240 260 280 300 320 340
Effect of Acetonitrile Concentration
p - Aminobenzoic Acid
COOH
NH
2
2–10% Acetonitrile
Wavelength
Absorbance
Absorbance
nM
Effect of pH
p -Aminobenzoic Acid
pH 3.1 pH 5.1 pH 6.9
COO–
Effect of Acetonitrile Concentration
p -Aminobenzoic Acid