32 Diagnostics and Calibration
2
To prepare for calibration:
1. Set the pump to deliver 1 mL/min of degassed methanol for 10 minutes. If methanol
is not miscible with the previous solvent, flush with a miscible sovent before
switching to methanol.
2. If you have been using buffers, flush with HPLC-quality water at 1 mL/min for 10
minutes, then switch to methanol for 10 minutes.
Note:
Ensure that the solvent is miscible with the previous mobile phase.
For information on performing calibration, refer to the “PDA Calibration Window” topic in
the
Millennium
32
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