6 Table of Contents
Chapter 3
Maintenance .................................................................................... 33
3.1 Flow Cell Maintenance.......................................................... 33
3.1.1 Flushing the Flow Cell ............................................... 33
3.1.2 Removing the Flow Cell............................................. 34
3.1.3 Disassembling and Cleaning the Flow Cell................ 36
3.1.4 Installing the Flow Cell Assembly .............................. 38
3.2 Replacing the Lamp.............................................................. 39
3.2.1 Checking Lamp Usage .............................................. 39
3.2.2 Removing the Lamp................................................... 41
3.3 Replacing the Fuses ............................................................. 43
Chapter 4
Principles of the 996 PDA Detector Optics ...................................... 44
4.1 996 Detector Optics .............................................................. 44
4.2 Resolving Spectral Data ....................................................... 46
4.3 Measuring Light at the Photodiode ....................................... 47
4.4 Computing Absorbance Data Points..................................... 50
4.4.1 Calculating Absorbance............................................. 50
4.4.2 Resolution.................................................................. 52
4.4.3 Filtering Data ............................................................. 53
Chapter 5
Spectral Contrast Theory ................................................................. 54
5.1 Comparing Absorbance Spectra........................................... 54
5.2 Representing Spectra as Vectors.......................................... 55
5.2.1 Vectors Derived from Two Wavelengths..................... 56
5.2.2 Vectors Derived from Multiple Wavelengths .............. 56