Suspected cause Possible solution
Needle weak wash solvent is too high in
organic.
Ensure that the weak needle wash solution is
similar to the initial mobile phase.
In-line filter, column inlet, or connecting tubing
is partially blocked.
Inspect these components for particle build-up.
Clean or replace.
Column or columns are contaminated. Replace columns.
See also: The appropriate column care and
use guide on the documentation CD.
Column or columns are degraded, forming a
void.
Verify column performance. If efficiency is low,
replace it.
Trap column is degraded. • Remove trap column and perform a direct
injection.
• If results are normal, replace the column
and perform a set pressure diagnostic.
4.3.2.13 Tailing peaks with a normal retention time
Suspected cause Possible solution
Fitting or interconnection problem Inspect sprayer and emitter plumbing
connections.
See also: The Universal NanoFlow Sprayer
Installation and Maintenance Guide on the
documentation CD.
Bad consumables Perform a systematic replacement of
consumables beginning with the analytical
column.
4.3.2.14 Tailing peaks with increased retention time
Suspected cause Possible solution
Leak in the system If the problem persists, perform the set-
pressure diagnostic test to inspect for system
leaks.
See also: The online Help for more
information.
November 26, 2019, 715003588 Revision C
Page 72
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