Table B–5: Mobile phase absorbance measured against air or water (continued)
Absorbance (AU) at specified wavelength (nm)
200 205 210 215 220 230 240 250 260 280
PIC D4, 1 vial/L 0.03 0.03 0.03 0.03 0.02 0.02 0.02 0.02 0.02 0.01
BRI J 35, 1% 0.06 0.03 0.02 0.02 0.02 0.01 <0.01 — — —
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate)
(CHAPS), 0.1%
2.40 2.32 1.48 0.80 0.40 0.08 0.04 0.02 0.02 0.01
Sodiumdodecyl sulfate (SDS), 0.1% 0.02 0.01 <0.01 — — — — — — —
4-octylphenol polyethoxylate (Triton X-100), 0.1% 2.48 2.50 2.43 2.42 2.37 2.37 0.50 0.25 0.67 1.42
Polyoxyethylene sorbitan monolaurate (Tween 20), 0.1% 0.21 0.14 0.11 0.10 0.09 0.06 0.05 0.04 0.04 0.03
August 8, 2016, 715005049 Rev. C
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