Waters ACQUITY - Page 99

Table B–5: Mobile phase absorbance measured against air or water (continued)
Absorbance (AU) at specified wavelength (nm)
200 205 210 215 220 230 240 250 260 280
Buffers and salts
Ammonium acetate, 10 mM 1.88 0.94 0.53 0.29 0.15 0.02 <0.01 — — —
Ammonium bicarbonate, 10 mM 0.41 0.10 0.01 <0.01 — — — — — —
Ethylenedinitrilotetraacetic acid disodium salt (disodium EDTA),
1 mM
0.11 0.07 0.06 0.04 0.03 0.03 0.02 0.02 0.02 0.02
4-(2-hydroxyethy l)-1-piperazineethanesulfonic acid (HEPES), 10
mM, pH 7.6
2.45 2.50 2.37 2.08 1.50 0.29 0.03 <0.01 — —
Morpholinoethanesulfonic acid (MES), 10 mM, pH 6.0 2.42 2.38 1.89 0.90 0.45 0.06 <0.01 — — —
Potassium phosphate, monobasic (KH
2
PO
4
), 10 mM 0.03 <0.01 — — — — — — — —
Potassium phosphate, dibasic, (K
2
HPO
4
), 10 mM 0.53 0.16 0.05 0.01 <0.01 — — — — —
Sodium acetate, 10 mM 1.85 0.96 0.52 0.30 0.15 0.03 <0.01 — — —
Sodium chloride, 1 M 2.00 1.67 0.40 0.10 <0.01 — — — — —
Sodium citrate, 10 mM 2.48 2.84 2.31 2.02 1.49 0.54 0.12 0.03 0.02 0.01
Sodium formate, 10 mM 1.00 0.73 0.53 0.33 0.20 0.03 <0.01 — — —
Sodium phosphate, 100 mM, pH 6.8 1.99 0.75 0.19 0.06 0.02 0.01 0.01 0.01 0.01 <0.01
Tris HCl, 20 mM, pH 7.0 1.40 0.77 0.28 0.10 0.04 <0.01 — — — —
Tris HCl, 20 mM, pH 8.0 1.80 1.90 1.11 0.43 0.13 <0.01 — — — —
Waters PIC reagents
PIC A, 1 vial/L 0.67 0.29 0.13 0.05 0.03 0.02 0.02 0.02 0.02 <0.01
PIC B6, 1 vial/L 2.46 2.50 2.42 2.25 1.83 0.63 0.07 <0.01 — —
PIC B6, low UV, 1 vial/L 0.01 <0.01 — — — — — — — —
August 8, 2016, 715005049 Rev. C
Page 99