Chapter 5 Analyze the Relative Standard Curve Experiment
Chapter Overview
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
76
Notes
Chapter Overview
The 7500 software analyzes the data using the relative standard curve quantitation
method. Section 1 of this chapter explains how to review the analyzed data using several
of the analysis screens and how to publish the data. Section 2. If you obtain questionable
results, Section 2 of this chapter explains how to perform some troubleshooting.
Note: The relative standard curve example experiment includes three flagged wells. The
flags indicate common problems that you may encounter when performing your own
experiments. Procedures are provided for viewing the flags and omitting one of the wells.
Example
Experiment
Workflow
Design the Experiment (Chapter 2)
Start Relative Standard Curve Experiment
Prepare the Reactions (Chapter 3)
Run the Experiment (Chapter 4)
End Experiment
Analyze the Experiment (Chapter 5)
Section 1, Review Results:
1. Analyze.
2. View the standard curve.
3. View the amplification plot.
4. View the gene expression plot/results table.
5. Publish the data.
Section 2, Troubleshoot (If Needed):
1. View the analysis settings; adjust the baseline/threshold.
2. View the quality summary.
3. Omit wells.
4. View the multicomponent plot.
5. View the raw data plot.
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