abi 7500 - Design the Relative Standard Curve Experiment

Chapter 2
19
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
Design the Relative Standard
Curve Experiment
This chapter covers:
■ Chapter Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
■ Create a New Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
■ Define the Experiment Properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
■ Define the Methods and Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
■ Set Up the Targets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
■ Set Up the Standards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
■ Set Up the Samples. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
■ Set Up the Relative Quantitation Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
■ Set Up the Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
■ Review the Reaction Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
■ Order Materials for the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
■ Finish the Design Wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
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