abi 7500 - View the Analysis Settings

Chapter 5 Analyze the Relative Standard Curve Experiment
View the Analysis Settings
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
100
Notes
View the Analysis Settings
The Analysis Settings dialog box displays the analysis settings for the threshold cycle
(C
T
), flags, and advanced options. If the default analysis settings in the 7500 software are
not suitable for your experiment, you can change the settings, then reanalyze your
experiment.
About the
Example
Experiment
In the standard curve example experiment, the default analysis settings are used without
changes.
View the Settings
1. In the navigation pane, select Analysis.
2. Click Analysis Settings to open the Analysis Settings dialog box.
3. In the example experiment, view the default analysis settings, which are specified in
each of the following tabs:
•C
T
Settings
• Flag Settings
• Advanced Settings
4. Click Apply Analysis Settings.