Chapter 4
65
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
Run the Relative Standard Curve
Experiment
This chapter covers:
■ Chapter Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
■ Prepare for the Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
■ Enable the Notification Settings (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
■ Start the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
■ Monitor the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
■ Unload the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Note: For more information about any of the topics discussed in this guide, open the
Help from within Applied Biosystems 7500/7500 Fast Real-Time PCR Software v2.0 by
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