Appendix A Alternate Experiment Workflows
QuickStart Workflow
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
206
Notes
QuickStart Workflow
When you create an experiment using QuickStart, you can run the reactions on the
instrument with no reaction plate setup information.
1. Prepare the PCR reactions:
2. QuickStart the experiment:
a. Double-click (7500 software) or select StartAll Programs
Applied Biosystems7500 Software<software name>
where <software name> is the current version of the 7500 software.
b. In the Home screen, click QuickStart.
c. Select the Experiment Properties tab (default), enter the experiment name,
then select the experiment properties.
d. Select the Run Method tab, review the reaction volume and thermal profile,
then edit as needed.
3. Run the experiment:
IMPORTANT! While the 7500/7500 Fast instrument is performing a run, do not
create experiments, perform maintenance, or allow the computer to run antivirus
software or to enter hibernation. Performing such activities while the instrument is
running an experiment will cause gaps in data collection.
a. Load the reaction plate into the instrument.
b. Start the run.
c. (Optional) Monitor the run.
d. Unload the reaction plate from the instrument.
Experiment Type Prepare the…
Relative standard curve a. Template.
b. Sample dilutions.
c. Standard dilution series.
d. Reaction mix.
e. Reaction plate.
Standard curve
Comparative C
T
a. Template.
b. Sample dilutions.
c. Reaction mix.
d. Reaction plate.
Genotyping
Presence/absence
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