abi 7500 - Prepare the Relative Standard Curve Reactions

Chapter 3
49
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
Prepare the Relative Standard
Curve Reactions
This chapter covers:
■ Chapter Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
■ Prepare the Template. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
■ Prepare the Sample Dilutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
■ Prepare the Standard Dilution Series . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
■ Prepare the Reaction Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
■ Prepare the Reaction Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
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