Chapter 5 Analyze the Relative Standard Curve Experiment
View the Multicomponent Plot
107
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
View the Multicomponent Plot
The Multicomponent Plot screen displays the complete spectral contribution of each dye
in a selected well over the duration of the PCR run.
About the
Example
Experiment
In the example experiment, you review the Multicomponent Plot screen for:
•ROX
™
dye (passive reference)
•FAM
™
dye (reporter)
• Spikes, dips, and/or sudden changes
• Amplification in the negative control wells
View the Plot
1. In the navigation pane, select Analysis Multicomponent Plot.
Note: If no data are displayed, click Analyze.
2. Display the unknown and standard wells one at a time in the Multicomponent Plot
screen:
a. Select the View Plate Layout tab.
b. Select one well in the plate layout; the well is shown in the Multicomponent
Plot screen.
Note: If you select multiple wells, the Multicomponent Plot screen displays the
data for all selected wells simultaneously.
3. In the Plot Color drop-down list, select Dye.
4. Click (Show a legend for the plot).
Note: This is a toggle button. When the legend is displayed, the button changes to
Hide the plot legend.
5. View the FAM dye signal. In the example experiment, the FAM dye signal increases
throughout the PCR process, which indicates normal amplification.
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