Chapter 5 Analyze the Relative Standard Curve Experiment
Omit Wells from the Analysis
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
106
Notes
5. Click Analyze to reanalyze the experiment data with well A9 removed from the
analysis.
Analysis
Guidelines
When you analyze your own relative standard curve experiment, carefully view the
replicate groups for outliers. If needed, remove outliers manually using the Well Table.
See “Omit Wells” on page 105 to remove the outliers in your experiment.
For More
Information
For more information on omitting wells from the analysis, open the 7500 Software Help
by clicking or pressing F1. Within the Help, search for the omit well topics:
1. Select the Search tab.
2. Enter omit well.
3. Click List Topics.
4. Double-click the topics you want to review.
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