Chapter 6 Design the Comparative C
T
Experiment
Order Materials for the Experiment
131
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
Order Materials for the Experiment
In the Materials List screen, review the list of materials recommended to prepare the PCR
reaction plate. (Optional) Print the materials list, create a shopping list, then order the
recommended materials from the Applied Biosystems Store.
Note: You must have an Internet connection to access the Applied Biosystems Store.
Product availability and pricing may vary according to your region or country. Online
ordering through the Applied Biosystems Store is not available in all countries. Contact
your local Applied Biosystems representative for help.
Note: The 7500 software recommends the materials to order based on your experiment
design. It is assumed that you will design your experiment, order your materials, then
prepare (Chapter 7) and run (Chapter 8) the reaction plate when your materials arrive.
About the
Example
Experiment
In the comparative C
T
example experiment, the recommended materials are:
•MicroAmp
®
Optical 96-Well Reaction Plate
•MicroAmp
™
Optical Adhesive Film
•MicroAmp
®
Splash Free Support Base
•TaqMan
®
Universal PCR Master Mix (2
✕
), No AmpErase
®
UNG
• 18S Assay Mix: Hs99999901_s1
• EGR3 Assay Mix: Hs00231780_m1 (RefSeq NM_004430.2)
• MAOB Assay Mix: Hs00168533_m1 (RefSeq NM_000898.3)
• OGDH Assay Mix: Mm00803121_m1 (RefSeq NM_010956.3)
• OSGEP Assay Mix: Hs00215099_m1 (RefSeq NM_017807.2)
• SERPING1 Assay Mix: Hs00163781_m1 (RefSeq NM_001032295.1/NM_000062.2)
Complete the
Ordering
Materials Screen
1. Find the target assay in the Applied Biosystems Store:
a. Click the Enter Gene Name field, enter 18S, then click Find Assay.
b. In the Find Assay Results dialog box, select the Hs99999901_s1 assay row,
then click Apply Assay Selection.
2. Repeat step 1 to apply the remaining target assays for the experiment:
• EGR3 Assay Mix: Hs00231780_m1
• MAOB Assay Mix: Hs00168533_m1
• OGDH Assay Mix: Mm00803121_m1
• OSGEP Assay Mix: Hs00215099_m1
• SERPING1 Assay Mix: Hs00163781_m1
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