abi 7500 - Order Materials for the Experiment

Chapter 2 Design the Relative Standard Curve Experiment
Order Materials for the Experiment
43
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
Order Materials for the Experiment
In the Materials List screen, review the list of materials recommended to prepare the PCR
reaction plate. You can print the materials list, create a shopping list, then order the
recommended materials from the Applied Biosystems Store.
Note: Product availability and pricing might vary according to your region or country.
Online ordering through the Applied Biosystems Store is not available in all countries.
Contact your local Applied Biosystems representative for help.
Note: The 7500 software recommends the materials to order based on your experiment
design. It is assumed that you will design your experiment, order your materials, then
prepare (Chapter 3) and run (Chapter 4) the reaction plate when your materials arrive.
About the
Example
Experiment
In the relative standard curve example experiment, the recommended materials are:
•MicroAmp
®
Optical 96-Well Reaction Plate
•MicroAmp
™
Optical Adhesive Film
•MicroAmp
®
Splash Free Support Base
•TaqMan
®
Universal PCR Master Mix (2✕), No AmpErase
®
UNG
• c-myc Assay Mix: Hs00153408_m1 (RefSeq NM_002467.3)
• GAPDH Assay Mix: Hs99999905_m1 (RefSeq NM_002046.3)
Complete the
Ordering
Materials Screen
1. Find the target assay in the Applied Biosystems Store:
a. Click the Enter Gene Name field, enter c-myc, then click Find Assay.
b. In the Find Assay Results dialog box, select the Hs00153408_m1 row, then
click Apply Assay Selection.
c. Click the Enter Gene Name field, enter GAPDH, then click Find Assay.
1b
1b