Chapter 9 Analyze the Comparative C
T
Experiment
Chapter Overview
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
156
Notes
Chapter Overview
The 7500 software analyzes the data using the comparative C
T
(∆∆C
T
) quantitation
method. This chapter includes the following sections:
• Section 9.1 explains how to review the analyzed data using several of the analysis
screens and how to publish the data.
• Section 9.2 explains how to perform some troubleshooting steps if you receive
questionable results.
• Section 9.3 explains how to analyze multiple experiments as a study.
Example
Experiment
Workflow
Analyze the Experiment (Chapter 9)
Section 1, Review Results:
1. Analyze.
2. View the Gene Expression Plot/well table.
3. View the Amplification Plot.
4. Publish the data.
Section 2, Troubleshoot (If Needed):
1. View the analysis settings; adjust the baseline/threshold.
2. View the quality summary.
3. Omit wells.
4. View the Multicomponent Plot.
5. View the Raw Data Plot.
Section 3, Create a Study:
1. Create a study.
2. Define replicates.
3. Analyze.
4. View the Gene Expression Plot.
5. View the experiment data.
6. View the Multicomponent Data.
7. View multiple plots.
8. View the quality summary.
9. Publish the data.
Design the Experiment (Chapter 6)
Start Comparative C
T
(∆∆C
T
) Experiment
Prepare the Reactions (Chapter 7)
Run the Experiment (Chapter 8)
End Experiment
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